Dr. Cynthia Haseltine
Room: BLS 137
Room: BLS 140
Research & Interests
I have two words for you:
Ok, maybe it should be more like nearly boiling acid, but I think you get the idea. It's harsh, it's dangerous, and it shouldn't support life. But it does. And not only can nearly boiling acid support life, it's practically teeming with it. Work in our lab is focused on organisms that shouldn't be able to live where they do and how they manage to live so very well. Specifically, we're interested in microscopic organisms called Archaea that are able to flourish in hot springs across the planet, as long as those spots are both very hot and very acidic. We're studying how these microbes survive in their hot springs, including ways they fix their DNA when it becomes damaged and what types of microbial warfare they use to compete in their environment. One aspect of our work is aimed toward understanding how cells resist DNA damage and repair broken DNA, both of which are important for unraveling the mechanisms behind cancer formation. A newer direction in the lab focuses on novel antimicrobials produced by Archaea which are promising alternatives for battling antibiotic resistant pathogens.
Fall and Summer
MBioS 404 Molecular Biology: Introduction of prokaryotic and eukaryotic genome organization and gene expression, modern molecular techniques, experimental approaches, genome and gene function and analyses.
MBioS 494 Capstone Senior Project in Molecular Biosciences: Written paper and seminar presentation on laboratory research project.
Dr. Haseltine's video interviews:
Graham, W., M. Rolfsmeier, and C. Haseltine. 2013. An archaeal RadA paralog influences presynaptic filament formation. DNA Repair.12:403-413.
Graham, W. and C. Haseltine. 2013. A recombinase paralog from the hyperthermophilic crenarchaeon Sulfolobus solfataricus enhances SsoRadA ssDNA binding and strand displacement. Gene. 515: 128-139.
C. Haseltine. Single-stranded DNA-Binding Proteins (SSBs). 2013. Brenner’s Encyclopedia of Genetics. Second Edition, Malloy, S. and K. Hughes, eds.
Rolfsmeier, M., M. Laughery, and C. Haseltine. 2011. Repair of DNA double-strand breaks induced by ionizing radiation damage correlates with upregulation of homologous recombination genes in Sulfolobus solfataricus. Journal of Molecular Biology. 414: 485-498.
Rolfsmeier, M., M. Laughery, and C. Haseltine. 2010. Repair of DNA double-strand breaks following UV damage in three Sulfolobus solfataricus strains. Journal of Bacteriology. 192(19): 4954-4962.
Rolfsmeier, M. and C. Haseltine. 2010. The single-stranded DNA binding protein of Sulfolobus solfataricus acts in the presynaptic step of homologous recombination. Journal of Molecular Biology. 397(1): 31-45.
Haseltine, C. and S. Kowalczykowski. 2009. An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA. Nucl. Acids Res., 37, 2757-2770.
Rolfsmeier, M. and C. Haseltine. 2008. Recombination processes and proteins in the Archaea. pp. 121-146 In: Archaea: New Models for Prokaryotic Biology. ed. P. Blum. Caister Academic Press, Norfolk, UK. 247 p.
Haseltine, C. and S. Kowalczykowski. 2002. A distinctive single-stranded DNA-binding protein from the Archaeon Sulfolobus solfataricus. Molecular Microbiology 43(6), 1505-1515.
Haseltine, C., Hill, T., Montalvo-Rodriguez, R., Kemper, S., Shand, R., and P. Blum. 2001. Secreted euryarchaeal microhalocins kill hyperthermophilic crenarchaea. Journal of Bacteriology 183(1), 287-291.
Seitz, E., Haseltine, C., and S. Kowalczykowski. 2001. DNA recombination and repair in the Archaea. Advances in Applied Microbiology 50, 101-169.
Montalvo-Rodriguez, R., Haseltine, C., Huess-LaRossa, K., Clemente, T., Soto, J., Staswick, P., and P. Blum. 2000. Autohydrolysis of plant polysaccharides using transgenic hyperthermophilic enzymes. Biotechnology and Bioengineering, 70(2) 151-159.
Haseltine, C., Montalvo-Rodriguez, R., Carl, A., Bini, E., and P. Blum. 1999. Extragenic pleiotropic mutations that repress glycosyl hydrolase expression in the hyperthermophilic archaeon Sulfolobus solfataricus. Genetics 152, 1353-1361.
Haseltine, C., Montalvo-Rodriguez, R., Bini, E., Carl, A., and P. Blum. 1999. Coordinate transcriptional control in the hyperthermophilic archaeon Sulfolobus solfataricus. Journal of Bacteriology 181(13), 3920-3927.
Rolfsmeier, M., Haseltine, C., Bini, E., Clark, A., and P. Blum. 1998. Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus. Journal of Bacteriology 180(5), 1287-1295.
Haseltine, C., Rolfsmeier, M., and P. Blum. 1996. The glucose effect and regulation of alpha-amylase synthesis in the hyperthermophilic archaeon Sulfolobus solfataricus. Journal of Bacteriology 178(4), 945-950.