Lisa M. Gloss
Interim Dean, Graduate School, and Coordinator of the Individual Interdisciplinary Degree Program
Room: BLS 133
Room: Room: BLS 140
Research & Interests
The Gloss lab uses biochemical and biophysical methods to characterize protein macromolecular assemblies, focusing on three systems: 1) folding of oligomeric histone proteins; 2) assembly of the core nucleosome; 3) haloadaptation of enzymes from the archae extreme halophiles. The overarching goal of these studies is to understand how protein sequence encodes structure, stability, assembly and function.
In the past two decades many protein folding studies have focused on small, single domain monomers, which fold by two-state kinetic mechanisms, with no transiently populated intermediates. These experimental studies, coupled with advanced computational approaches, have suggested that folding intermediates may be kinetic traps which hinder productive folding. Focusing on the canonical histone hand-shake dimerization motif, we are providing an essential extension of protein folding studies to oligomeric proteins, and asking how does a protein’s primary structure encode its secondary, tertiary and quaternary structure and the mechanism of efficient formation of intra- and intermolecular contacts to yield the native three-dimensional structure. We have found a positive correlation between the population of kinetic intermediates and faster dimerization and folding, suggesting the hypothesis that, for larger proteins, kinetic intermediates accelerate productive folding. We are testing this hypothesis with WT and mutant forms of the eukaryotic heterodimers, H2A-H2B and H3-H4, and two archael homodimeric histones, hMfB and hPyA1
The nucleosome core particle (NCP) is the fundamental repeating unit of DNA compaction into chromosome. The dynamic nature of the NCP is an important regulator of many DNA-templated chemistries: transcription, replication, recombination and repair. Misregulation of these chemistries leads to a range of diseases, particularly cancer. Our long-term goal is to understand how histone alternations (sequence differences in histone variants or post-translational modifications) and interactions (with histone chaperones or chromatin remodeling complexes) regulate NCP stability, assembly and dynamics. Our current focus is development and utilization of protein-protein Förster Resonance Energy Transfer (FRET) system to monitor the equilibrium and kinetic processes of H2A-H2B dimer dissociation from the H3-H4 tetramer & DNA binary complex.
Halophilic and mesophilic dihydrofolate reductases.
The goal of this research is to determine the sequence determinants that permit function of enzymes from the archae extreme halophiles at high ionic strength (2 to 4 M) and correspondingly lower water activity. The results will elucidate how the aqueous solvent and ionic cosolutes influence the stability, solubility and catalytic activity of enzymes—information that is essential for the engineering of better biocatalysts for a range of biotechnological applications. We use the well-characterized E. coli dihydrofolate reductase (ecDHFR) as our mesophilic model for comparison to the DHFRs from Haloferax volcanii (hvDHFR1 and hvDHFR2). The ionic strength dependence of several properties of the WT and mutant DHFRs are being examined: equilibrium stability, substrate affinity, catalytic activity and enzyme solubility.
- Chen, Yujie, Tokuda, Joshua M., Topping, Traci B., Meisburger, Steve P., Pabit, Suzette A., Gloss, Lisa M. & Pollack, Lois (2017) Asymmetric unwrapping of nucleosomal DNA propogates asymmetric opening and dissociation of the histone core. Proc. Natl Acad Sci USA. 114: 334-9.
- Chen, Yujie, Tokuda, Joshua M., Topping, Traci, Sutton, Julie L., Meisburger, S.P., Pabit, Suzette A., Gloss, Lisa M. & Pollack, Lois (2014) Revealing transient structures of nucleosomes as DNA unwinds. Nucleic Acids Res. 42, 8767-76
- Guyett, Paul J. and Gloss, Lisa M. (2012) The H2A-H2B dimeric kinetic intermediate is stabilized by wide-spread hydrophobic burial with few fully native interactions. J. Mol. Biol. 415, 600-614
- Topping, Traci B. & Gloss, Lisa M. (2011) The impact of solubility and electrostatics on fibril formation by the H3 and H4 histones. Protein Science. 20, 2060-72
- Stump, Matthew R. & Gloss, Lisa M. (2010) Mutational studies uncover non-native structure in the dimeric kinetic intermediate of the H2A-H2B heterodimer. J. Mol. Biol. 401, 518-531 PMCID: PMC2914809
- Gloss, Lisa M. (2009) Equilibrium and kinetic approaches for studying oligomeric protein folding. Methods Enzymol. 466,d 325-357
- Stump MR, Gloss LM. (2008) Mutational analysis of the stability of the H2A and H2B histone monomers. J Mol. Bio. 384(5), 1369-83 PMID: 18976667 PMCID: PMC2644352
- Gloss, Lisa M., Topping, Traci B., Binder, April K. & Lohman, Jeremy R. (2008) Kinetic folding of Haloferax volcanii and Escherichia coli dihydrofolate reductases: Haloadaptation by unfolded state destabilization at high ionic strength. J. Mol. Biol. 376, 1451-1462 PMID: 18207162
- Stump MR, Gloss LM. (2008) Unique Fluorophores in the Dimeric Archaeal Histones hMfB and hPyA1 Reveal the Impact of Non-native Structure in a Monomeric Kinetic Intermediate. Protein Sci. 17(2), 322-32 PMID: 18096639 PMCID: PMC2222717
- Hoch DA, Stratton JJ, Gloss LM. (2007) Protein-protein Förster Resonance Energy Transfer Analysis of Nucleosome Core Particles Containing H2A and H2A.Z J Mol. Biol. 371(4), 971-88 PMID: 17597150 PMCID: PMC2031861
- Placek BJ, Harrison LN, Villers BM, Gloss LM. (2005) The H2A.Z-H2B dimer is very unstable in comparison to the dimer with H2B formed by the dominant H2A variant. Protein Sci. 14(2), 514-22 PMID: 15632282 PMCID: PMC2253418
- Placek BJ, Gloss LM. (2005) Three-state kinetic folding mechanism of the H2A/H2B histone heterodimer: the N-terminal tails affect the transition state between a dimeric intermediate and the native dimer. J Mol. Biol. 345(4), 827-36 PMID: 15588829
- Topping TB, Gloss LM. (2004) The differential stabilities of histones from mesophilic, thermophilic and hyperthermophilic archae are manifested in both the rates of folding and unfolding. J Mol. Biol. 342(1), 247-60 PMID: 15313621
- Banks DD, Gloss LM. (2004) Folding of the H3-H4 dimer and assembly into the (H3-H4)2 tetramer. Protein Sci 13(5), 1304-16 PMID: 15096635 PMCID: PMC2286770
- Topping TB, Hoch DA, Gloss LM. (2004) Folding mechanism of FIS, the intertwined, helical Factor for Inversion Stimulation. J Mol. Biol. 335(4), 1065-81 PMID: 14698300
- Banks DD, Gloss LM. (2003) The equilibrium folding of the core histones: The H3-H4 tetramer is less stable than the H2A-H2B dimer. Biochemistry. 42(22), 6827-39 PMID: 12779337
- Gloss LM, Placek BJ. (2002) The effects of salt on the stability of the H2A-H2B dimer Biochemistry. 41(50), 14951-9 PMID: 12475244
- Placek BJ, Gloss LM. (2002) The N-terminal tails of the H2A-H2B affect dimer structure and stability. Biochemistry. 41(50), 14960-8 PMID: 12475245
- Wright DB, Banks DD, Lohman JR, Hilsenbeck JL, Gloss LM. (2002) Comparison of the effects of salt on the activity and stability of Escherichia coli and Haloferax volcanii dihydrofolate reductases. J Mol. Biol. 323(2), 327-44 PMID: 12381324
- Gloss LM, Simler BR, Matthews CR (2001) Rough energy landscapes in protein folding: Dimeric E. coli Trp repressor folds through three parallel channels J Mol. Biol. 312(5), 1121-34. PMID: 11580254
- Mevarech M, Frolow F, Gloss LM. (2000) Halophilic Enzymes: Proteins with a Grain of Salt. Biophys Chem. PMID: 11026680