SMB Faculty Profiles

John J. Wyrick

John J. Wyrick

Room: BLS 241
Office Phone: 509-335-8785
Room: BLS 240
Lab Phone: 509-335-8787

Lab Website



Research & Interests

We are investigating how DNA damage is formed and repaired in chromatin on a genome-wide scale, and how damage formation and repair influences subsequent mutagenesis. Our focus has been on UV-induced DNA damage, which is primarily repaired by nucleotide excision repair (NER) pathway. We are also investigating how nucleosome positioning and histone modifications regulate the repair of DNA base lesions throughout the genome. To advance these studies, we have developed novel genomic tools to map DNA lesions at single-nucleotide resolution throughout the yeast and human genomes. We have also been investigating how chromatin impacts CRISPR-Cas9 genome editing.

Select Publications
  • Mao P, Wyrick JJ, Roberts SA, Smerdon MJ (2017) UV-Induced DNA Damage and Mutagenesis in Chromatin. Photochem Photobiol. 93(1), 216-228 PMID: 27716995 PMCID: PMC5315636
  • Choi JA, Wyrick JJ. (2017) RegulatorDB: a resource for the analysis of yeast transcriptional regulation Database (Oxford). 2017. doi: 10.1093/database/bax058. PMID: 29220449 PMCID: PMC5737240
  • Mao P, Brown AJ, Malc EP, Mieczkowski PA, Smerdon MJ, Roberts SA, Wyrick JJ. (2017) Genome-wide maps of alkylation damage, repair, and mutagenesis in yeast reveal mechanisms of mutational heterogeneity Genome Res. 27(10):1674-1684. doi: 10.1101/gr.225771.117. Epub 2017 Sep 14. PMID: 28912372 PMCID: PMC5630031
  • Hodges AJ, Gloss LM, Wyrick JJ. (2017) Residues in the Nucleosome Acidic Patch Regulate Histone Occupancy and Are Important for FACT Binding in Saccharomyces cerevisiae Genetics 206(3):1339-1348. doi: 10.1534/genetics.117.201939. Epub 2017 May 3. PMID: 28468903 PMCID: PMC5500134
  • Kong M, Liu L, Chen X, Driscoll KI, Mao P, Böhm S, Kad NM, Watkins SC, Bernstein KA, Wyrick JJ, Min JH, Van Houten B (2016) Single-molecule imaging reveals that Rad4 (XPC) employs a dynamic DNA damage recognition process. Mol Cell. 64(2), 376-387 PMID: 27720644 PMCID: PMC5123691
  • Mao P, Wyrick JJ (2016) Emerging Roles for Histone Modifications in DNA Excision Repair FEMS Yeast Research 16(7) PMID: 27737893 PMCID:
  • Mao P, Smerdon MJ, Roberts SA, Wyrick JJ (2016) Chromosomal landscape of UV damage formation and repair at single-nucleotide resolution. Pro Natl Acad Sci USE. 113(32), 9057-62 PMID: 27457959 PMCID: PMC4987812
  • Rodriguez Y, Hinz JM, Laughery MF, Wyrick JJ, Smerdon MJ (2016) Site-specific Acetylation of Histone H3 Decreases Polymerase β Activity on Nucleosome Core Particles in Vitro. J Biol Chem. 291(21), 11434-45 PMID: 27033702 PMCID: PMC4900286
  • Wyrick, JJ, Roberts SA (2015) Genomic approaches to DNA repair and mutagenesis. DNA repair 36, 146-55 PMID: PMCID:
  • Meas R, Smerdon MJ, Wyrick JJ (2015) The amino-terminal tails of histones H2A and H3 coordinate efficient base excision repair, DNA damage signaling and postreplication repair in Saccharomyces cerevisiae. Nucleic Acids Res 43(10), 4990-5001 PMID: 25897129 PMCID: PMC4446432
  • Hodges AJ, Gallegos IJ, Laughery MF, Meas R, Tran L, Wyrick JJ (2015) Histone Sprocket Arginine Residues Are Important for Gene Expression, DNA Repair, and Cell Viability in Saccharomyces cerevisiae. Genetics 200(3), 795-806 PMID: 25971662 PMCID: PMC4512544
  • Zavala AG, Morris RT, Wyrick JJ, Smerdon MJ (2014) High-resolution characterization of CPD hotspot formation in human fibroblasts. Nucleic Acid Res. 42(2), 893-905 PMID: 24137003 PMCID: PMC3902913
  • Wyrick JJ, Kyriss MN, Davis WB (2012) Ascending the nucleosome face: recognition and function of structured domains in the histone H2A-H2B dimer
    Biochim Biophys Acta. 1819(8), 892-901 PMID: 22521324 PMCID: PMC3461831
  • Kyriss MN, Jin Y, Gallegos IJ, Sanford JA, Wyrick JJ (2010) Novel functional residues in the core domain of histone H2B regulate yeast gene expression and silencing and affect the response to DNA damage Mol Cell Biol. 30(14), 3503-18 PMID: 20479120 PMCID: PMC2897548
  • Nag R, Kyriss M, Smerdon JW, Wyrick JJ, Smerdon MJ (2010) A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast Nucleic Acids Res. 38(5), 1450-60 PMID: 20007597 PMCID: PMC2836547
  • Jin Y, Rodriguez AM, Wyrick JJ (2009) Genetic and Genome-wide Analysis of Simultaneous Mutations in Acetylated and Methylated Lysine Residues in Histone H3 in Saccharomyces cerevisiae Genetics 181(2), 461-72 PMID: 19087956 PMCID: PMC2644940
  • Chaudhuri S, Wyrick JJ, Smerdon MJ (2009) Histone H3 Lys79 Methylation is Required for Efficient Nucleotide Excision Repair in a Silenced Locus of Saccharomyces cerevisiae Nucleic Acids Res. 37(5), 1690-700 PMID: 19155276 PMCID: PMC2655692
  • Wyrick JJ, Parra MA (2009) The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim Biophys Acta. 1789(1), 37-44 PMID: 18675384 PMCID:
  • Parra MA, Wyrick JJ (2007) Regulation of gene transcription by the histone H2A N-terminal domain. Mol Cell Biol 27(21), 7641-8 PMID: 17724083 PMCID: PMC2169066
  • Wood A, Shukla A, Schneider J, Lee JS, Stanton JD, Dzuiba T, Swanson SK, Florens L, Washburn MP, Wyrick J, Bhaumik SR, Shilatifard A (2007) Ctk complex mediated regulation of histone methylation by COMPASS Mol Cell Biol. 27(2), 709-20 PMID: 17088385 PMCID: PMC1800791
  • Parra MA, Kerr D, Fahy D, Pouchnik DJ, Wyrick JJ (2006) Deciphering the Roles of the Histone H2B N-terminal domain in Genome-wide Transcription Mol Cell Biol. 26(10), 3842-52 PMID: 16648479 PMCID: PMC1489011
Washington State University